E-ISSN 2576-3288
 

Original Research (Original Article) 


Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike.

Abstract
Objective: Costus afer Ker Gawl is an African ethno-medicinal plant used as a therapeutic for the treatment of oxidative stress-related diseases. This study was designed to identify the most active fractions of C.afer leaves and stem against calcium(II) (Ca2+)-induced mitochondrial membrane permeability transition (MPT).
Methods: C.afer leaves and stem were extracted using 70% methanol and subsequently subjected to successive solvent partitioning method to separate it into hexane, ethyl acetate, n-butanol and aqueous fraction of C.afer leaves and stem. Rat liver mitochondrial fractions were isolated using differential centrifugation technique, and mitochondrial MPT was assessed using a spectrophotometric method.
Results: Data showed that n-butanol fractions of C.afer leaves and stem exhibited a significantly high inhibition of Ca2+-induced mitochondrial MPT than other test fractions and standard spermine.
Conclusion: n-butanol fractions of Costus afer leaves and stem possess bioactive compounds with chemoprotective activity against Ca2+-induced mitochondrial MPT. Furthermore, it is suggested that n-butanol fractions of C.afer leaves and stem could be harnessed for bioactive compounds that can be used in the treatment of mitochondria-associated neurodegenerative diseases.

Key words: Bioactive compounds; calcium ion; Costus afer; mitochondria


 
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Pubmed Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. Am J Res Med Sci. 2017; 1(1): 21-26. doi:10.5455/ajrms.281653


Web Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. http://www.ajrms.com/?mno=281653 [Access: December 09, 2018]. doi:10.5455/ajrms.281653


AMA (American Medical Association) Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. Am J Res Med Sci. 2017; 1(1): 21-26. doi:10.5455/ajrms.281653



Vancouver/ICMJE Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. Am J Res Med Sci. (2017), [cited December 09, 2018]; 1(1): 21-26. doi:10.5455/ajrms.281653



Harvard Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike (2017) Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. Am J Res Med Sci, 1 (1), 21-26. doi:10.5455/ajrms.281653



Turabian Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. 2017. Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. American Journal of Research in Medical Sciences, 1 (1), 21-26. doi:10.5455/ajrms.281653



Chicago Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. "Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition." American Journal of Research in Medical Sciences 1 (2017), 21-26. doi:10.5455/ajrms.281653



MLA (The Modern Language Association) Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike. "Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition." American Journal of Research in Medical Sciences 1.1 (2017), 21-26. Print. doi:10.5455/ajrms.281653



APA (American Psychological Association) Style

Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike (2017) Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition. American Journal of Research in Medical Sciences, 1 (1), 21-26. doi:10.5455/ajrms.281653





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