E-ISSN 2576-3288
 

Original Research 


Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.

zubair labu, Jalal Uddin, Abu Jafor Biswas.

Abstract
Aim: To find out the biological activities of thrombolytic, membrane stabilizing activity and also quantification of total phenol content in vitro model from the leave extract of Butea monosperma (B. monosperma)
Method: The collected leave was dried and grinded. The grinded powder was kept in 2500 ml of 90% methanol for several days followed by filtered. At reduced temperature 40° C, the volume of filtrated crude methanolic extract was reduced by a vacuum rotary evaporator under reduced pressure. Following the concentrated aqueous methanol extractive was partitioned into petroleum ether, carbon tetrachloride, chloroform and aqueous soluble fractions respectively by the Kupchan methods, Then all extracts were subjected to evaluate in vivo thrombolytic by Daginawala, membrane stabilizing properties by Okoli and Omale method and total phenol content followed by the method of Wolfe et al.
Result: In thrombolytic assay among the extractives, petroleum soluble fraction showed the highest percent clot lysis (27.36 ± 0.10%) activity as compared with standard streptokinase (65.15 ± 0.16%). In case of membrane stabilizing activity, crude methanolic extract profoundly inhibited the lysis of erythrocytes (65.79 ± 0.40%) induced by osmosis, whereas reference standard acetyl salicylic acid resulted in (83.147 ± 0.39%) inhibition of lysis. On the other hand, aqueous soluble fraction showed slightly higher level of membrane stabilizing activity in the heat-induced hemolysis (75.3 ± 0.39). In the total phenolic content test, petroleum soluble fraction was found significant level of phenolic (249.06 mg of GAE/gm).

Conclusions: This study was conducted to validate the B. monosperma leaves used as a folk medicine (for the ailment of thrombosis, inflammation) such as thrombolytic, membrane stabilizing

Key words: Butea monosperma; thrombolytic; membrane stabilizing activity; total phenolic content.


 
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Pubmed Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. Am J Res Med Sci. 2018; 2(2): 58-65. doi:10.5455/ajrms.283065


Web Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. http://www.ajrms.com/?mno=283065 [Access: December 09, 2018]. doi:10.5455/ajrms.283065


AMA (American Medical Association) Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. Am J Res Med Sci. 2018; 2(2): 58-65. doi:10.5455/ajrms.283065



Vancouver/ICMJE Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. Am J Res Med Sci. (2018), [cited December 09, 2018]; 2(2): 58-65. doi:10.5455/ajrms.283065



Harvard Style

zubair labu, Jalal Uddin, Abu Jafor Biswas (2018) Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. Am J Res Med Sci, 2 (2), 58-65. doi:10.5455/ajrms.283065



Turabian Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. 2018. Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. American Journal of Research in Medical Sciences, 2 (2), 58-65. doi:10.5455/ajrms.283065



Chicago Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. "Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.." American Journal of Research in Medical Sciences 2 (2018), 58-65. doi:10.5455/ajrms.283065



MLA (The Modern Language Association) Style

zubair labu, Jalal Uddin, Abu Jafor Biswas. "Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.." American Journal of Research in Medical Sciences 2.2 (2018), 58-65. Print. doi:10.5455/ajrms.283065



APA (American Psychological Association) Style

zubair labu, Jalal Uddin, Abu Jafor Biswas (2018) Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model.. American Journal of Research in Medical Sciences, 2 (2), 58-65. doi:10.5455/ajrms.283065





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