Evaluation of hepatoprotective activity of Allium sativum ethanolic extract in thioacetamide-induced hepatotoxicity in albino Wistar rats
Krishna Mohan Chinnala, Praisy Pinky Jayagar, Gayathri Motta, Raghuma Chowdary Adusumilli, Madhan Mohan Elsani
Aim: The present study was designed to evaluate the hepatoprotective effect of ethanolic extract of Allium sativum (EEAS) on thioacetamide (TAA)-induced hepatotoxicity in rats.
Methods: Male Wistar rats were administered 200 and 400 mg/kg/p.o. of EEAS for 21 days and simultaneously administered TAA 50 mg/kg/s.c. 1 hour after the respective assigned treatments for every 72 hours. At the end of experimental methods, all the animals were sacrificed by cervical decapitation. Blood samples were collected. Serum was separated and analyzed for various parameters like aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), and total bilirubin using commercially available test kits.
Results: Results indicate that EEAS administration blunt TAA-induced increase in activities of different marker enzymes of hepatocellular injury, viz., AST, ALT, ALP, and total bilirubin significantly (p < 0.001), suggesting that EEAS possibly has a protective influence against TAA-induced hepatocellular injury and degenerative changes.