Preventive activities against thrombosis and inflammation of Butea monosperma (Lam.) leaves methanolic extract in vitro model
Jalal Uddin, Abu Jafor Bishwash, Zubair Khalid Labu
Aim: To find out the biological activities such as thrombolytic, membrane stabilizing activity, and also quantification of total phenol content in vitro model from the leaves extracts of Butea monosperma.
Method: The collected leaves were dried and ground. About 250 g of powdered leaves were soaked in 2.5 L (1:2.5 w/v) of 90% methanol for 14 days at room temperature, 25 ± 1°C with irregular shaking. The dark brown color medium was filtered using a filter funnel and hand-held mechanical vacuum pump. The filtrate was dried using rotary evaporator at 120–160 rpm low pressure and temperature of 40°C. The recovered solvent was reused for three successive refluxes carried after every 24 hours of additional soaking. Following the concentration, crude methanol extract (CME) was partitioned into petroleum ether (PESF), carbon tetrachloride (CTSF), chloroform (CSF), and aqueous soluble fractions (AQSF), respectively, by the Kupchan methods. Then all extracts were subjected to evaluate in vitro thrombolytic by Daginawala, membrane stabilizing activity by Okoli and Omale, and total phenol content followed by Folin-Ciocalteu methods.
Result: In thrombolytic assay among the extractives, petroleum soluble fraction showed the highest percentage of clot lysis (27.36 ± 0.10%) activity while the standard streptokinase exhibited the lysis of clot by 65.15 ± 0.16%. In case of membrane stabilizing activity, crude methanolic extract profoundly inhibited the lysis of erythrocyte membrane (65.79 ± 0.40%) induced by osmosis, whereas reference standard acetyl salicylic acid resulted in (83.147 ± 0.39%) inhibition of lysis. On the other hand, AQSF showed slightly higher level of membrane stabilizing activity in the heat-induced hemolysis (75.3 ± 0.39). The petroleum soluble fraction was found to have significant level of total phenolic content (249.06 mg of GAE/gm).
Conclusion: This study was conducted to validate the B. monosperma leaves used as a folk medicine for the ailment of thrombosis, inflammation such as thrombolytic and membrane stabilizing.