E-ISSN 2576-3288
 

Original Research (Original Article) 


Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko.

Abstract
Aim: Chromolaena odorata has been used traditionally for the treatment of various conditions which include skin infections, wounds, ulcers and also used as an antimalarial. The present work investigates the ability of the methanolic extract of the plant to inhibit hydrogen-peroxide induced haemolysis and lipid peroxidation of human erythrocytes. Other in vitro antioxidant and free radical scavenging activities were also investigated.
Materials and Methods: Dried leaves of Chromolaena odorata were pulverized and extracted using absolute methanol. Veinous blood was collected from a healthy individual and erythrocytes isolated. Erythrocyte haemolysis and lipid peroxidation were induced using hydrogen peroxide and subsequently treated with Chromolaena odorata extract. The plant extract was also assessed for its hydrogen peroxide scavenging, hydroxyl radical scavenging and reducing abilities. In all cases, vitamin C was used as the reference compound.
Results: The results show that Chromolaena odorata extract reduced hydrogen peroxide-induced haemolysis and lipid peroxidation when compared to vitamin C. While the IC50 values for the extract were 4.11 mg/mL and 12.95 mg/mL for inhibition of erythrocyte haemolysis and lipid peroxidation respectively, the IC50 values for vitamin C were 1.98 mg/mL and 0.57 mg/mL for haemolysis and lipid peroxidation. The extract also possessed hydrogen peroxide scavenging, hydroxyl radical scavenging and reducing abilities. In most cases, the effects were concentration-dependent and significant among various extracts concentrations (p < 0.05).
Conclusion: The observed responses indicate that the plant possessed immense antioxidant potential which is ascribed to the bioactive phytochemicals. This could be exploited pharmacologically.

Key words: Chromolaena odorata, lipid peroxidation, haemolysis, reducing ability, antioxidant


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Angela Nwachukwu
Articles by Chioma Peace Iyagbo
Articles by Juliet Nneoma Onyenweaku
Articles by Tebekeme Okoko
on Google
on Google Scholar
Article Statistics
 Viewed: 1173
Downloaded: 394
Cited: 0

How to Cite this Article
Pubmed Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. Am J Res Med Sci. 2018; 2(1): 17-23. doi:10.5455/ajrms.281421


Web Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. http://www.ajrms.com/?mno=281421 [Access: December 09, 2018]. doi:10.5455/ajrms.281421


AMA (American Medical Association) Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. Am J Res Med Sci. 2018; 2(1): 17-23. doi:10.5455/ajrms.281421



Vancouver/ICMJE Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. Am J Res Med Sci. (2018), [cited December 09, 2018]; 2(1): 17-23. doi:10.5455/ajrms.281421



Harvard Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko (2018) Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. Am J Res Med Sci, 2 (1), 17-23. doi:10.5455/ajrms.281421



Turabian Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. 2018. Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. American Journal of Research in Medical Sciences, 2 (1), 17-23. doi:10.5455/ajrms.281421



Chicago Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. "Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis." American Journal of Research in Medical Sciences 2 (2018), 17-23. doi:10.5455/ajrms.281421



MLA (The Modern Language Association) Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko. "Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis." American Journal of Research in Medical Sciences 2.1 (2018), 17-23. Print. doi:10.5455/ajrms.281421



APA (American Psychological Association) Style

Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko (2018) Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis. American Journal of Research in Medical Sciences, 2 (1), 17-23. doi:10.5455/ajrms.281421





Most Viewed Articles
  • Juvenile rectal polyp with osseous metaplasia- A rare case report.
    Sonam S Nandyal, Krishnamurthy P Shashikala, Lakmapur Math Manjunath
    Am J Res Med Sci. 2017; 1(1): 35-37
    » Abstract » doi: 10.5455/ajrms.281681

  • Primary Intracranial Myxoid Chondrosarcoma: A case report
    Hayam E. Rashed, Aziza E. Abdelrahman, Wael Elmesallamy, Ahmed A.E. Obaya
    Am J Res Med Sci. 2018; 2(1): 44-47
    » Abstract » doi: 10.5455/ajrms.281691

  • Effect of antioxidant rich nutraceuticals on histological changes due to high fat diet induced obesity in rats
    Suleiman A. Isa, Muazu G. Abubakar, Muhammed S. Abubakar, Muhammad A. Ubana, Abdullahi Dandare
    Am J Res Med Sci. 2017; 1(1): 8-13
    » Abstract » doi: 10.5455/ajrms.281722

  • Effect of edible camphor administrations on levels of steroid and thyroid hormones in male wistar rats
    Oluwatobi T Somade, Regina N Ugbaja, Abdullahi A Adebayo
    Am J Res Med Sci. 2017; 1(1): 27-34
    » Abstract » doi: 10.5455/ajrms.281388

  • Study of Keratinocyte Proliferation, Apoptosis and Dermal Inflammation in Psoriatic Skin Lesions Before and After Methotrexate Therapy: Which Change Is Contributing More to Clinical Severity of the Disease?
    Amany Abdel Bary, Ahmed Abdel Bary
    Am J Res Med Sci. 2018; 2(1): 34-43
    » Abstract » doi: 10.5455/ajrms.281926

  • ROLE OF MELATONIN IN AMELIORATING CYTOTOXIC EFFECT OF GOLD NANOPARTICLES ON THE PULMONARY ALVEOLI OF ADULT ALBINO RATS.
    Ehab Mostafa Elzawawy, Abeer Gaber Ahmed
    Am J Res Med Sci. 2018; 2(1): 24-33
    » Abstract » doi: 10.5455/ajrms.281645

  • Organ-Specific Effects of Selenium Glutathione Peroxidases in Response to Medicinal Plant Extracts
    Janka Vašková, Gabriela Moj& 382;išová, Ladislav Vaško, Michal Stanko, Janka Porá& 269;ová
    Am J Res Med Sci. 2018; 2(1): 1-8
    » Abstract » doi: 10.5455/ajrms.281449

  • Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition
    Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike
    Am J Res Med Sci. 2017; 1(1): 21-26
    » Abstract » doi: 10.5455/ajrms.281653

  • Relevance of a new HPLC assay for 4-hydroxy-2-nonenal in human plasma
    Andrea Bolner, Ottavio Bosello, Giampietro Nordera
    Am J Res Med Sci. 2017; 1(1): 1-7
    » Abstract » doi: 10.5455/ajrms.281669

  • Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis
    Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko
    Am J Res Med Sci. 2018; 2(1): 17-23
    » Abstract » doi: 10.5455/ajrms.281421

  • Most Downloaded
  • Effect of antioxidant rich nutraceuticals on histological changes due to high fat diet induced obesity in rats
    Suleiman A. Isa, Muazu G. Abubakar, Muhammed S. Abubakar, Muhammad A. Ubana, Abdullahi Dandare
    Am J Res Med Sci. 2017; 1(1): 8-13
    » Abstract » doi: 10.5455/ajrms.281722

  • Primary Intracranial Myxoid Chondrosarcoma: A case report
    Hayam E. Rashed, Aziza E. Abdelrahman, Wael Elmesallamy, Ahmed A.E. Obaya
    Am J Res Med Sci. 2018; 2(1): 44-47
    » Abstract » doi: 10.5455/ajrms.281691

  • ROLE OF MELATONIN IN AMELIORATING CYTOTOXIC EFFECT OF GOLD NANOPARTICLES ON THE PULMONARY ALVEOLI OF ADULT ALBINO RATS.
    Ehab Mostafa Elzawawy, Abeer Gaber Ahmed
    Am J Res Med Sci. 2018; 2(1): 24-33
    » Abstract » doi: 10.5455/ajrms.281645

  • Study of Keratinocyte Proliferation, Apoptosis and Dermal Inflammation in Psoriatic Skin Lesions Before and After Methotrexate Therapy: Which Change Is Contributing More to Clinical Severity of the Disease?
    Amany Abdel Bary, Ahmed Abdel Bary
    Am J Res Med Sci. 2018; 2(1): 34-43
    » Abstract » doi: 10.5455/ajrms.281926

  • An in vitro screening study of food functions among vegetable cultivars consumed in Japan
    Naoki Ohkura, Riko Fujita, Ruri Kanai, Kenichi Ishibashi, Hiroshi Ueda, Gen-ichi Atsumi
    Am J Res Med Sci. 2018; 3(1): 1-6
    » Abstract » doi: 10.5455/ajrms.20180126090406

  • Effect of edible camphor administrations on levels of steroid and thyroid hormones in male wistar rats
    Oluwatobi T Somade, Regina N Ugbaja, Abdullahi A Adebayo
    Am J Res Med Sci. 2017; 1(1): 27-34
    » Abstract » doi: 10.5455/ajrms.281388

  • Acute and sub-chronic oral toxicity studies on the methanol leaf extract of Gnetum africanum Welw in wistar rats.
    Nkeiruka Emmanuela Udeh, Aruh Anaga, Isaac Uzoma Asuzu
    Am J Res Med Sci. 2018; 3(1): 7-14
    » Abstract » doi: 10.5455/ajrms.20180109094114

  • Organ-Specific Effects of Selenium Glutathione Peroxidases in Response to Medicinal Plant Extracts
    Janka Vašková, Gabriela Moj& 382;išová, Ladislav Vaško, Michal Stanko, Janka Porá& 269;ová
    Am J Res Med Sci. 2018; 2(1): 1-8
    » Abstract » doi: 10.5455/ajrms.281449

  • Antioxidant evaluation and inhibitory activity of Chromolaena odorata leaf extract on hydrogen peroxide-induced erythrocyte hemolysis
    Angela Nwachukwu, Chioma Peace Iyagbo, Juliet Nneoma Onyenweaku, Tebekeme Okoko
    Am J Res Med Sci. 2018; 2(1): 17-23
    » Abstract » doi: 10.5455/ajrms.281421

  • Paracetamol and Hydrogen peroxide-induced tissue alterations are reduced by Costus afer extract
    Tebekeme Okoko
    Am J Res Med Sci. 2018; 2(2): 66-72
    » Abstract » doi: 10.5455/ajrms.20171230100944

  • Most Cited Articles