American Journal of Research in Medical Sciences. 2017; 1(1):(3-42)
Bioactive compounds in Costus afer Ker Gawl leaves and stem fractions protect against calcium ion-induced mitochondrial membrane permeability transition
Godswill N. Anyasor, Efemena E. Obukohwo, Olanipekun O. Adenike
Methods: C.afer leaves and stem were extracted using 70% methanol and subsequently subjected to successive solvent partitioning method to separate it into hexane, ethyl acetate, n-butanol and aqueous fraction of C.afer leaves and stem. Rat liver mitochondrial fractions were isolated using differential centrifugation technique, and mitochondrial MPT was assessed using a spectrophotometric method.
Results: Data showed that n-butanol fractions of C.afer leaves and stem exhibited a significantly high inhibition of Ca2+-induced mitochondrial MPT than other test fractions and standard spermine.
Conclusion: n-butanol fractions of Costus afer leaves and stem possess bioactive compounds with chemoprotective activity against Ca2+-induced mitochondrial MPT. Furthermore, it is suggested that n-butanol fractions of C.afer leaves and stem could be harnessed for bioactive compounds that can be used in the treatment of mitochondria- associated neurodegenerative diseases.